全文获取类型
收费全文 | 258篇 |
免费 | 6篇 |
国内免费 | 2篇 |
出版年
2016年 | 5篇 |
2015年 | 5篇 |
2013年 | 8篇 |
2012年 | 4篇 |
2011年 | 10篇 |
2010年 | 13篇 |
2009年 | 12篇 |
2008年 | 9篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 11篇 |
2004年 | 5篇 |
2003年 | 6篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 9篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 8篇 |
1980年 | 10篇 |
1979年 | 4篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1969年 | 1篇 |
1966年 | 1篇 |
1957年 | 1篇 |
1956年 | 2篇 |
1951年 | 2篇 |
1947年 | 1篇 |
排序方式: 共有266条查询结果,搜索用时 31 毫秒
31.
The use of vesicular-arbuscular mycorrhizal roots grown by the nutrient film technique as inoculum for field sites 总被引:2,自引:0,他引:2
R. P. ELMES CHRISTINE M. HEPPER D. S. HAYMAN J. O'SHEA 《The Annals of applied biology》1984,104(3):437-441
Bean roots grown by the nutrient film technique and infected with vesicular-arbuscular mycorrhizal fungi were successfully used as inoculum for red clover growing in an unsterile field site. The endophyte introduced with the roots became well established in the presence of indigenous mycorrhizal fungi. 相似文献
32.
CHRISTINE GABRIELIDES VASSILIOS M. KAPOULAS 《The Journal of eukaryotic microbiology》1981,28(4):441-447
ABSTRACT. [14C]chimyl and [3H]batyl alcohols were added to Crithidia fasciculata cultures during the mid-log phase of cell growth, and the lipid extracts of the cells were analyzed for degradation products. C. fasciculata cells were able to take up exogenous glyceryl ethers, and in amounts as high as the endogenous lipid content. The glyceryl ether taken up by the cells was incorporated into lipids either prior to the ether bond cleavage or after degradation to fatty acid. The extent of degradation and the degree of incorporation of degradation products into cellular lipid were higher for chimyl than for batyl alcohol. Batyl alcohol was not metabolized efficiently, leading to the formation of large intracellular pools of free substrate. One product of glyceryl ether degradation was identified as alkyl-dihydroxy acetone, and was detected inside and outside of the cells. The data strongly suggest that this product is the first stable intermediate in the degradation process and indicate that the extracellular formation of alkyl-dihydroxy acetone is due to the action of exocnzyme; ecteted by the cells. The constant detection of alk I cnyl glycerol among the degradation products indicates the existence of a second mechantsm in C. fasciculata for converting the alkyl-to alkenyl-glycerol. 相似文献
33.
Physiological Changes in the Hemiparasite Rhinanthus serotinus before and after Attachment 总被引:2,自引:0,他引:2
Growth of the hemiparasite Rhinanthus serotinus (Schönh.) Oborny was greatly stimulated after attachment of the parasite to the roots of the host plant, Hordeum vulgare L. In order to find the limiting factors for the growth of Rhinanthus without a host, unattached and attached Rhinanthus plants were compared. Within I day after attachment the contents of nitrogen, phosphorus, potassium, magnesium, and sodium increased considerably. Organic nitrogen and phosphorus compounds were rapidly synthesized in attached Rhinanthus. The accumulation of sugars in unattached Rhinanthus and the decrease in sugar content after attachment suggested that the main requirement from the host was not for carbohydrates. 相似文献
34.
D. M. MOORE J. B. HARBORNE CHRISTINE A. WILLIAMS 《Botanical journal of the Linnean Society. Linnean Society of London》1970,63(4):277-293
Thirty-one flavonoids (and related phenolics) were found in leaves of the six species in the three genera of Empetraceae (90 populations studied) and five further pigments in fruits of Empetrum. Affinity with the Ericaceae is supported by the occurrence of the 3-galactosidesof gossypetin, malvidin, delphinidin, cyanidin and peonidin. The distribution of leaf flavonoids agrees with both the recognition of three genera and species-delimitation in Ceratiola and Corema. In Empetrum , the flavonoids differ modally in the three species usually accepted, thus confirming data on fruit colour and breeding systems, and suggesting that the genus comprises one species with about three subspecies at diploid and tetraploid levels. The black fruits of most Northern Hemisphere plants contain malvidin, petunidin and delphinidin, while the red fruits of almost all Southern Hemisphere populations ( E. rubrum ) contain cyanidin and peonidin and provide a rare example of the association of cyanidin (instead of delphinidin) with the wild-type allele.
In leaf flavonoid content the diploid E. nigrum is much closer to its presumed tetraploid derivative E. eamesii than to the diploid E. rubrum , which differs most from E. eamesii. Flavonoid patterns and breeding system distinguish most E. rubrum populations from those isolated in Tristan da Cunha-Gough Island. The phytogeographical importance of the phytOGhemical results is discussed. 相似文献
In leaf flavonoid content the diploid E. nigrum is much closer to its presumed tetraploid derivative E. eamesii than to the diploid E. rubrum , which differs most from E. eamesii. Flavonoid patterns and breeding system distinguish most E. rubrum populations from those isolated in Tristan da Cunha-Gough Island. The phytogeographical importance of the phytOGhemical results is discussed. 相似文献
35.
ABSTRACT Earlier studies showed that Leishmania major promastigotes are sensitive to osmotic conditions. A reduction in osmolality caused the cells to shorten and to rapidly release most of their large internal pool of alanine. In this study some effects of hyper-osmotic stress were examined. an increase in osmolality of the culture medium from 308 to 625 mOsm/kg caused only a small decrease in growth rate. When cells grown in the usual culture medium (308 mOsm/kg) were washed, resuspended in iso-osmotic buffer, and subjected to acute hyper-osmotic stress by addition of mannitol, the alanine content increased even in the absence of exogenous substrate. Promastigotes, depleted of alanine by a 5-min exposure to hypo-osmotic conditions, also synthesized alanine when resuspended in iso-osmotic buffer. Washed cells resuspended in iso-osmotic buffer consume their internal pool of alanine under aerobic conditions, Rates of consumption decreased on addition of mannitol, becoming zero at about 440 mOsm/kg. At higher osmolalities, alanine synthesis occurred. to estimate whether proteolysis could account for alanine synthesis in the absence of exogenous substrate, cells that had been grown with [1-14 C]leucine were washed and resuspended under hypo-, iso-, and hyper-osmotic conditions and the amounts of 14 CO2 and 14 C-labelled peptides released in 1 h were measured. Little proteolysis occurred under these conditions, but the possibility that proteolysis was the source of the alanine increase, observed in response to hyper-osmotic stress, cannot be ruled out. 相似文献
36.
37.
38.
39.
G. A. GILBERT LILO M. GILBERT CHRISTINE E. OWENS NAHED A. F. SHAWKY 《Nature: New biology》1972,235(56):110-112
ACCURATE measurement of the energy of protein subunit interaction1–7 is an essential step in the study of cooperative and allosteric8–10 effects in proteins. It is, however, more often the change of this energy on uptake of ligand or substrate which is the more important quantity to be determined, not its total value. Likewise it is the variation of interaction energy from protein to protein which is of more interest when phylogenetically related proteins are compared and again it is differences in energy of combination which determine how much hybrid is formed when closely related reversibly dissociating proteins are present in mixture in solution. 相似文献
40.
SHAUN L. WINTERTON NATE B. HARDY STEPHEN D. GAIMARI MARTIN HAUSER HILARY N. HILL KEVIN C. HOLSTON MICHAEL E. IRWIN CHRISTINE L. LAMBKIN MARK A. METZ FEDERICA TURCO LONGLONG YANG DAVID K. YEATES BRIAN M. WIEGMANN 《Systematic Entomology》2016,41(1):144-161
The therevoid clade represents a group of four families (Apsilocephalidae, Evocoidae, Scenopinidae and Therevidae) of lower brachyceran Diptera in the superfamily Asiloidea. The largest of these families is that of the stiletto flies (Therevidae). A large‐scale (i.e. supermatrix) phylogeny of Therevidae is presented based on DNA sequence data from seven genetic loci (16S, 18S and 28S ribosomal DNA and four protein‐encoding genes: elongation factor 1‐alpha, triose phosphate isomerase, short‐wavelength rhodopsin and the CPSase region of carbamoyl‐phosphate synthase‐aspartate transcarbamoylase‐dihydroorotase). Results are presented from Bayesian phylogenetic analyses of approximately 8.7 kb of sequence data for 204 taxa representing all subfamilies and genus groups of Therevidae. Our results strongly support the sister‐group relationship between Therevidae and Scenopinidae, with Apsilocephalidae as sister to Evocoidae. Previous estimates of stiletto fly phylogeny based on morphology or DNA sequence data, or supertree analysis, have failed to find significant support for relationships among subfamilies. We report for the first time strong support for the placement of the subfamily Phycinae as sister to the remaining Therevidae, originating during the Mid Cretaceous. As in previous studies, the sister‐group relationship between the species‐rich subfamilies Agapophytinae and Therevinae is strongly supported. Agapophytinae are recovered as monophyletic, inclusive of the Taenogera group. Therevinae comprise the bulk of the species richness in the family and appear to be a relatively recent and rapid radiation originating in the southern hemisphere (Australia + Antarctica + South America) during the Late Cretaceous. Genus groups are defined for all subfamilies based on these results. 相似文献